IL-8 anticorps

N° du produit ABIN115766

L’anticorps Souris Monoclonal anti-IL-8 a été validé pour EIA et IHC (fro). Il convient pour détecter IL-8 dans des échantillons de Humain.

Aperçu rapide pour IL-8 anticorps (ABIN115766)

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-8 est non-conjugé

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SB-84-8

Détail du produit anti-IL-8 anticorps

Purification: Protein G Affinity Chromatography

Immunogène: Highly purified recombinant Human IL-8

Isotype: IgG1

Information d'application

Indications d'application: ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect at least 1000 pg/mL of recombinant Human IL-8 when used withbiotinylated antigen affinity purified anti-Human IL-8 (cat. no. PP1030B) as the detectionantibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human IL-8, this antibody can be used at a concentration of0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for one month and at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Date de péremption: 12 months

Détails sur IL-8

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Autre désignation: Interleukin-8 / IL8

Sujet: IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

ID gène: 9606

UniProt: P10145

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy